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mouse vegf duoset elisa kit (R&D Systems)

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R&D Systems mouse vegf duoset elisa kit
Mouse Vegf Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+vegf+duoset+elisa+kit/Mouse+VEGF+DuoSet+ELISA/pm41899867-152-1-7
Average 95 stars, based on 153 article reviews

mouse vegf duoset elisa kit - by Bioz Stars, 2026-10

95/100 stars

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Enzyme-linked Immunosorbent Assay:

Article Title: Establishment of a 3D Multicellular HCC Tumor Spheroid Model to Unravel Nrf2’s Influence on the Tumor Immune Microenvironment
Article Snippet: Cell culture supernatant was harvested after six days of spheroid formation for Enzyme-linked immunosorbent assay (ELISA). .. The Mouse VEGF DuoSet ® ELISA kit (#DY493, R&D Systems, Minneapolis, MN, USA) was used according to the manufacturer’s prescribed protocol; 100 μL of undiluted supernatant was used. .. The plate was analyzed with the Infinite M200 Microplate Reader (Tecan Life Sciences, Männedorf, Switzerland).

Article Title: Metalloproteinase inhibition reduces AML growth, prevents stem cell loss, and improves chemotherapy effectiveness
Article Snippet: .. Legend MAXTM Mouse CXCL12 (SDF-1ß) ELISA kit (Biolegend, Cat # 444207), SCF (KITL) Mouse ELISA kit (Thermofisher, Cat. # EMKITL), Mouse VEGF DuoSet ELISA kit (R&D system, Cat #DY493), Mouse Thrombopoietin (TPO/THPO) ELISA kit (Thermofisher, Cat #EMTHPO), Mouse TGF beta 1- ELISA kit (Abcam, #Ab119557) and Mouse PF4 (CXCL4) ELISA kit (Abcam, #Ab202403) were used according to the manufacturer’s instructions. .. Intravital microscopy (IVM) was performed using a Zeiss LSM 780 upright confocal microscope equipped with Argon (458, 488 and 514 nm), a diode-pumped solid-state 561 nm laser and a Helium-Neon 633 nm, a tunable infrared multiphoton laser (Spectraphysics Mai Tai DeepSee 690-1200nm), 4 non-descanned detectors (NDD) and an internal spectral detector array.

Article Title: Signaling events at TMEM doorways provide potential targets for inhibiting breast cancer dissemination
Article Snippet: .. ELISA was performed as per the manufacturer’s recommendation using the mouse VEGF DuoSet ELISA kit from R&D Systems. ..

Article Title: Nrf2 promotes reparative angiogenesis through regulation of NADPH oxidase-2 in oxygen-induced retinopathy
Article Snippet: .. The protein concentrations of VEGF and TNF-α were assessed using Mouse VEGF DuoSet ELISA kit (R&D Systems) and Mouse TNF-α DuoSet ELISA kit (R&D Systems) as previously described [ 48 ]. .. Briefly, retinas were ultrasonically homogenized in 0.1% Triton X-100 in PBS containing a cocktail of protease inhibitors (Life Technologies).

Article Title: Establishment of a 3D Multicellular HCC Tumor Spheroid Model to Unravel Nrf2's Influence on the Tumor Immune Microenvironment.
Article Snippet: Cell culture supernatant was harvested after six days of spheroid formation for Enzyme-linked immunosorbent assay (ELISA). .. The Mouse VEGF DuoSet® ELISA kit (#DY493, R&D Systems, Minneapolis, MN, USA) was used according to the manufacturer’s prescribed protocol; 100 μL of undiluted supernatant was used. .. The plate was analyzed with the Infinite M200 Microplate Reader (Tecan Life Sciences, Männedorf, Switzerland). https://doi.org/10.3390/bioengineering13030336

Article Title: The transcriptional control of the VEGFA-VEGFR1 (FLT1) axis in alternatively polarized murine and human macrophages.
Article Snippet: .. Protein levels of VEGFA and FLT1 were determined using Mouse VEGF DuoSet ELISA Kit (R&D Systems; DY493) and Mouse VEGFR1/FLT-1 Quantikine ELISA Kit (R&D Systems; MVR100), in accordance with the instructions provided by the manufacturer. ..

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Tumor tEVs induce VEGF production from macrophages. (A) Overall scheme to investigate tumor tEV‐mediated VEGF production from macrophages. (B), (C) C57BL/6 mice were subcutaneously introduced with Matrigel (0.5 mL) containing PBS or tumor tEVs (10 µg). After 7 days, Matrigel was extracted, and paraffin sections of the Matrigel were immunostained with anti‐VEGF (in green) as well as anti‐F4/80 (in red) antibodies, and counter‐stained with Hoechst (in blue). (B) Images of paraffin sections stained for F4/80, VEGF, and Hoechst are shown. Scale bars represent 100 µm. (C) Fluorescence intensities of F4/80 staining were measured in VEGF‐negative or ‐positive areas within section images ( n = 6). (D) C57BL/6 mice were subcutaneously introduced with Matrigel (0.5 mL) containing PBS or tumor tEVs (10 µg). After 7 days, total RNA was isolated from Matrigel, and the level of VEGF mRNA was analyzed using real‐time RT‐PCR ( n = 3). Values represent the ratios of VEGF mRNA/GAPDH mRNA normalized to those in Matrigel containing PBS. (E) VEGF protein level in the conditioned media of peritoneal macrophages treated with a varying concentration of tumor tEVs for 24 h was measured by ELISA ( n = 4). Data are presented as mean ± SD. ** p < 0.01; and *** p < 0.001, calculated by two‐way ANOVA (C), unpaired Student's t ‐test (D), and one‐way ANOVA (E). Bonferroni correction for multiple comparisons was also performed for one‐ and two‐way ANOVA.

Journal: Journal of Extracellular Vesicles

Article Title: Mouse Tumor Tissue‐Derived Extracellular Vesicles Induce Angiogenesis Through VEGF Production From Macrophages

doi: 10.1002/jev2.70138

Figure Lengend Snippet: Tumor tEVs induce VEGF production from macrophages. (A) Overall scheme to investigate tumor tEV‐mediated VEGF production from macrophages. (B), (C) C57BL/6 mice were subcutaneously introduced with Matrigel (0.5 mL) containing PBS or tumor tEVs (10 µg). After 7 days, Matrigel was extracted, and paraffin sections of the Matrigel were immunostained with anti‐VEGF (in green) as well as anti‐F4/80 (in red) antibodies, and counter‐stained with Hoechst (in blue). (B) Images of paraffin sections stained for F4/80, VEGF, and Hoechst are shown. Scale bars represent 100 µm. (C) Fluorescence intensities of F4/80 staining were measured in VEGF‐negative or ‐positive areas within section images ( n = 6). (D) C57BL/6 mice were subcutaneously introduced with Matrigel (0.5 mL) containing PBS or tumor tEVs (10 µg). After 7 days, total RNA was isolated from Matrigel, and the level of VEGF mRNA was analyzed using real‐time RT‐PCR ( n = 3). Values represent the ratios of VEGF mRNA/GAPDH mRNA normalized to those in Matrigel containing PBS. (E) VEGF protein level in the conditioned media of peritoneal macrophages treated with a varying concentration of tumor tEVs for 24 h was measured by ELISA ( n = 4). Data are presented as mean ± SD. ** p < 0.01; and *** p < 0.001, calculated by two‐way ANOVA (C), unpaired Student's t ‐test (D), and one‐way ANOVA (E). Bonferroni correction for multiple comparisons was also performed for one‐ and two‐way ANOVA.

Article Snippet: Quantification of mouse VEGF protein was performed using DuoSet ELISA kit (R&D Systems) according to the manufacturer's protocol.

Techniques: Staining, Fluorescence, Isolation, Quantitative RT-PCR, Concentration Assay, Enzyme-linked Immunosorbent Assay

Validation of EMMPRIN expression in CT26-KD cells. The parental CT26 cells (CT26-WT) were infected with the lentivirus vector carrying EMMPRIN siRNA sequences (CT26-KD), or with the empty vector as negative control (CT26-NC). All three cells were each plated in 24-well plates (8×10 4 cells/well/300μL) in full medium for 48 h. Total RNA or protein were extracted from the cells and supernatants were collected. (A) EMMPRIN mRNA was amplified and determined by qPCR (n=4). (B) Expression of membranal EMMPRIN was determined by flow cytometry (n=3). Grey line, isotype control; blue line, CT26-WT; light blue, CT26-NC; red line, CT26-KD cells. (C) . EMMPRIN protein expression in cell lysates (n=4), and (D) Secreted EMMPRIN levels in supernatants were evaluated by ELISA (n=9). Data are presented as means ± SEM and are analyzed using one-way ANOVA followed by the Bonferroni’s post hoc test. (E) Representative western blot analysis of EMMPRIN expression in CT26-WT and CT26-KD cells, and (F) its quantitation (n=5). Data are presented as means ± SEM, and analyzed using the unpaired two-tailed student t test.

Journal: Frontiers in Immunology

Article Title: EMMPRIN promotes spheroid organization and metastatic formation: comparison between monolayers and spheroids of CT26 colon carcinoma cells

doi: 10.3389/fimmu.2024.1374088

Figure Lengend Snippet: Validation of EMMPRIN expression in CT26-KD cells. The parental CT26 cells (CT26-WT) were infected with the lentivirus vector carrying EMMPRIN siRNA sequences (CT26-KD), or with the empty vector as negative control (CT26-NC). All three cells were each plated in 24-well plates (8×10 4 cells/well/300μL) in full medium for 48 h. Total RNA or protein were extracted from the cells and supernatants were collected. (A) EMMPRIN mRNA was amplified and determined by qPCR (n=4). (B) Expression of membranal EMMPRIN was determined by flow cytometry (n=3). Grey line, isotype control; blue line, CT26-WT; light blue, CT26-NC; red line, CT26-KD cells. (C) . EMMPRIN protein expression in cell lysates (n=4), and (D) Secreted EMMPRIN levels in supernatants were evaluated by ELISA (n=9). Data are presented as means ± SEM and are analyzed using one-way ANOVA followed by the Bonferroni’s post hoc test. (E) Representative western blot analysis of EMMPRIN expression in CT26-WT and CT26-KD cells, and (F) its quantitation (n=5). Data are presented as means ± SEM, and analyzed using the unpaired two-tailed student t test.

Article Snippet: Concentrations of the mouse EMMPRIN in cell supernatants (diluted 1:100) were determined using the matched antibody pair kit (Abcam), and those of MMP-9 and VEGF (supernatants diluted 1:100) were evaluated with the DuoSet ELISA kits (R&D Systems, Minneapolis, MN).

Techniques: Biomarker Discovery, Expressing, Infection, Plasmid Preparation, Negative Control, Amplification, Flow Cytometry, Control, Enzyme-linked Immunosorbent Assay, Western Blot, Quantitation Assay, Two Tailed Test

Reduced EMMPRIN expression inhibits the angiogenic potential. (A) CT26-WT, CT26-KD and CT26-NC cells were seeded in serum starvation medium (8x10 4 cells/well/300μL) in 24-well plates for 48 h, their supernatants were collected, diluted (1:2) and applied onto a scratched monolayer of the bEND3 mouse endothelial cells. (B) Alternatively, CT26-WT, CT26-KD and CT26-NC cells (5x10 3 cells/well/100μL) were seeded in 96-well plates coated with 1% agarose, allowed to form spheroids for 3 days, and their collected supernatants were used in the same manner on bEND3 cells. (A, B) Representative images were taken at the start of the experiment (0h) and after 24 hours (24h). Bar size for monolayers and spheroids is 150μM. (C) The distance that the cells migrated to in order to close the gap was measured (n=7 for monolayers, n=8 for spheroids). The concentrations of (D) VEGF (n=7 for monolayers, n=8 for spheroids), and (E) MMP-9 (n=7 for monolayers, n=7 for spheroids) were measured by ELISA. Data are presented as means ± SEM, and analyzed by one-way ANOVA followed by Bonferroni’s post-hoc test.

Journal: Frontiers in Immunology

Article Title: EMMPRIN promotes spheroid organization and metastatic formation: comparison between monolayers and spheroids of CT26 colon carcinoma cells

doi: 10.3389/fimmu.2024.1374088

Figure Lengend Snippet: Reduced EMMPRIN expression inhibits the angiogenic potential. (A) CT26-WT, CT26-KD and CT26-NC cells were seeded in serum starvation medium (8x10 4 cells/well/300μL) in 24-well plates for 48 h, their supernatants were collected, diluted (1:2) and applied onto a scratched monolayer of the bEND3 mouse endothelial cells. (B) Alternatively, CT26-WT, CT26-KD and CT26-NC cells (5x10 3 cells/well/100μL) were seeded in 96-well plates coated with 1% agarose, allowed to form spheroids for 3 days, and their collected supernatants were used in the same manner on bEND3 cells. (A, B) Representative images were taken at the start of the experiment (0h) and after 24 hours (24h). Bar size for monolayers and spheroids is 150μM. (C) The distance that the cells migrated to in order to close the gap was measured (n=7 for monolayers, n=8 for spheroids). The concentrations of (D) VEGF (n=7 for monolayers, n=8 for spheroids), and (E) MMP-9 (n=7 for monolayers, n=7 for spheroids) were measured by ELISA. Data are presented as means ± SEM, and analyzed by one-way ANOVA followed by Bonferroni’s post-hoc test.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Reduced EMMPRIN expression enhances dormancy. CT26 tumor cells were grown as described before, in monolayers or in spheroids. (A) Representative images of staining for the expression of vimentin in monolayers by immunofluorescence (bar size is 25μm) and in spheroids by immunohistochemistry (bar size is 50μm). (B) Quantitation of vimentin expression (n=7 for monolayers, n=8 for spheroids). (C) Lysates were extracted from the cells, and the concentrations of phosphorylated p38 and ERK1/2 were determined by ELISA. The ratio pERK/pP38 was calculated (n=6 for both monolayers and spheroids). (D-J) Total RNA was extracted from monolayers or spheroids, transcribed to cDNA and amplified using primers for EMT-TFs or dormancy markers ( <xref ref-type=

Table 1 ). (D) NR2F1 (n=5 for monolayers and spheroids). (E) p21 (n=4 for monolayers and spheroids). (F) SOX2 (n=6 for monolayers and spheroids). (G) Snail (n=5 for monolayers and spheroids). (H) Slug (n=5 for monolayers and spheroids). (I) Twist1 (n=4 for monolayers and spheroids). (J) Zeb1 (n=4-5 for monolayers and spheroids). Data are presented as means ± SEM, and analyzed by one-way ANOVA followed by Bonferroni’s post-hoc test. " width="100%" height="100%">

Journal: Frontiers in Immunology

Article Title: EMMPRIN promotes spheroid organization and metastatic formation: comparison between monolayers and spheroids of CT26 colon carcinoma cells

doi: 10.3389/fimmu.2024.1374088

Figure Lengend Snippet: Reduced EMMPRIN expression enhances dormancy. CT26 tumor cells were grown as described before, in monolayers or in spheroids. (A) Representative images of staining for the expression of vimentin in monolayers by immunofluorescence (bar size is 25μm) and in spheroids by immunohistochemistry (bar size is 50μm). (B) Quantitation of vimentin expression (n=7 for monolayers, n=8 for spheroids). (C) Lysates were extracted from the cells, and the concentrations of phosphorylated p38 and ERK1/2 were determined by ELISA. The ratio pERK/pP38 was calculated (n=6 for both monolayers and spheroids). (D-J) Total RNA was extracted from monolayers or spheroids, transcribed to cDNA and amplified using primers for EMT-TFs or dormancy markers ( Table 1 ). (D) NR2F1 (n=5 for monolayers and spheroids). (E) p21 (n=4 for monolayers and spheroids). (F) SOX2 (n=6 for monolayers and spheroids). (G) Snail (n=5 for monolayers and spheroids). (H) Slug (n=5 for monolayers and spheroids). (I) Twist1 (n=4 for monolayers and spheroids). (J) Zeb1 (n=4-5 for monolayers and spheroids). Data are presented as means ± SEM, and analyzed by one-way ANOVA followed by Bonferroni’s post-hoc test.

Techniques: Expressing, Staining, Immunofluorescence, Immunohistochemistry, Quantitation Assay, Enzyme-linked Immunosorbent Assay, Amplification

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